Journal: Cell Reports Medicine

Article Title: A humanized monoclonal antibody targeting an ectonucleotidase rescues cardiac metabolism and heart function after myocardial infarction

doi: 10.1016/j.xcrm.2024.101795

Figure Lengend Snippet:

Article Snippet: Von Kossa Stain Kit , StatLab , KTVKO.

Techniques: Purification, Recombinant, cDNA Synthesis, Staining, RNA Sequencing, Cloning, Software

Journal: Nature Communications

Article Title: Na + -H + exchanger 1 determines atherosclerotic lesion acidification and promotes atherogenesis

doi: 10.1038/s41467-019-11983-3

Figure Lengend Snippet: Acidic human and murine atherosclerotic lesions are rich in macrophages, IgE expression, and cell apoptosis. a pHrodo-positive acidic area, CD68-positive macrophages, IgE, and TUNEL-positive cells are colocalized in parallel sections from human atherosclerotic lesions (data are representative from five donors). b Parallel section hematoxylin and eosin staining and von Kossa staining for tissue morphology and calcification. A human atherosclerotic lesion section with calcification is used as a von Kossa staining positive control. c In parallel atherosclerotic lesion sections from Apoe –/– mice, the pHrodo-positive acidic area (red) are rich in Mac-3-positive macrophages. d IgE expression and TUNEL-positive cells from the same area of panel c . e pHrodo is replaced with water as negative control in atherosclerotic lesion from Apoe –/– mice. Data are representative from seven mice. Bars: 500 µm, inset bars: 250 µm

Article Snippet: Serial histologic sections of the human atherosclerotic lesions were also stained with von Kossa staining using a von Kossa stain kit (Cat# KTVKOPT, American MasterTech, LODI, CA) to detect plaque calcification.

Techniques: Expressing, TUNEL Assay, Staining, Positive Control, Negative Control

Journal: BMJ Open Diabetes Research & Care

Article Title: Expression of the NEK7/NLRP3 inflammasome pathway in patients with diabetic lower extremity arterial disease

doi: 10.1136/bmjdrc-2020-001808

Figure Lengend Snippet: Ultrasound, H&E staining and Von Kossa staining of artery of patients with diabetic foot. The bar represents 200 µm. ATA, arteriae tibialis anterior; Con, control group (n=5); DLEAD, diabetic lower extremity arterial disease (n=12).

Article Snippet: Von Kossa staining was performed to examine calcification of the arteries according to the manufacturer’s instructions (Von Kossa staining kit, Solarbio, Beijing, China), and calcium staining of the arteries was analysed by light microscopy.

Techniques: Staining, Control